A novel In-111-labeled antisense DNA probe with multi-chelating sites (MCSprobe) showing high specific radioactivity and labeling efficiency

Citation
Y. Fujibayashi et al., A novel In-111-labeled antisense DNA probe with multi-chelating sites (MCSprobe) showing high specific radioactivity and labeling efficiency, NUCL MED BI, 26(1), 1999, pp. 17-21
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
NUCLEAR MEDICINE AND BIOLOGY
ISSN journal
0969-8051 → ACNP
Volume
26
Issue
1
Year of publication
1999
Pages
17 - 21
Database
ISI
SICI code
0969-8051(199901)26:1<17:ANIADP>2.0.ZU;2-A
Abstract
A multi-aminolinked oligodeoxynucleotide (ODN) was synthesized by substitut ion of dT with aminolinked dU in the sequence, following conjugation with i sothiocyanobenzyl-EDTA (IBE) for In-111 labeling. As a model target gene, t he c-erbB-2 protooncogene was used. The probability of the number of aminol inked dU in the 20mer ODN was 5, but there were actually 3 and 4 in the sel ected antisense and sense ODNs, respectively. The IBE/ODN conjugation level s of probes with multi chelating sites (MCS-probe) were 1.6 (antisense) and 2.4 (sense), more than 50 times higher than those of our previous studies using 5'-end aminolinked ODNs (IBE/ODN = 0.03), Labeling studies using the MCS probe and In-111 indicated that specific radioactivity as high as 48 MB q/nmol could be obtained with a labeling efficiency of over 90%. The In-111 antisense MCS-probe could bound to sense ODN under physiological condition s, but the In-111-sense-MCA-probe could not. Thus, side-chain modification of ODN for metal labeling is considered to be useful for antisense techniqu es. NUCL MED BIOL 26;1:17-21, 1999. (C) 1998 Elsevier Science Inc.