Single cell gel electrophoresis analysis of genomic damage induced by ethyl methanesulfonate in cultured tobacco cells

Citation
Da. Stavreva et al., Single cell gel electrophoresis analysis of genomic damage induced by ethyl methanesulfonate in cultured tobacco cells, MUT RES-F M, 422(2), 1998, pp. 323-330
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
ISSN journal
1386-1964 → ACNP
Volume
422
Issue
2
Year of publication
1998
Pages
323 - 330
Database
ISI
SICI code
1386-1964(199812)422:2<323:SCGEAO>2.0.ZU;2-2
Abstract
A procedure for employing cultured tobacco cells (line TX1) in the SCGE ass ay was developed. The effect on DNA migration was studied in control and EM S-treated cells at different stages in their growth curve. The experimental parameters of treatment time and the unwinding time were analyzed in TX1 c ells. With EMS in a concentration range from 0 to 30 mM the average median (+/-S.E.) tail moments ranged from 1.84 +/- 0.36 mu m for the negative cont rols and increased in a direct concentration dependent manner to 44.47 +/- 1.90 for cells treated with 30 mM EMS. Nuclei isolated from TX1 cells and t reated with EMS had a similar sensitivity as TX1 cells after EMS treatment. The plant cells express similar concentration-response curves for EMS as r eported with mammalian (CHO) cells. This plant cell SCGE assay may prove to be a useful tool for the study of agricultural chemicals in specific plant cell types, to compare the response of mutagens in plant and animal cells and for basic research in genetic toxicology and DNA repair in plants. (C) 1998 Elsevier Science B.V. All rights reserved.