Infection-derived Enterococcus faecalis strains are enriched in esp, a gene encoding a novel surface protein

Citation
V. Shankar et al., Infection-derived Enterococcus faecalis strains are enriched in esp, a gene encoding a novel surface protein, INFEC IMMUN, 67(1), 1999, pp. 193-200
Citations number
50
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
INFECTION AND IMMUNITY
ISSN journal
0019-9567 → ACNP
Volume
67
Issue
1
Year of publication
1999
Pages
193 - 200
Database
ISI
SICI code
0019-9567(199901)67:1<193:IEFSAE>2.0.ZU;2-F
Abstract
We report the identification of a new cell wall-associated protein of Enter ococcus faecalis, Studies on the distribution of the gene encoding this nov el surface protein, Esp, reveal a significant (P < 0.001) enrichment in inf ection-derived E. faecalis isolates. Interestingly, the esp gene was not id entified in any of 34 clinical E, faecium isolates or in I other less patho genic enterococcal species tested. Analysis of the structural gene among va rious E,faecalis isolates reveals the existence of alternate forms of expre ssion of the Esp protein. The deduced primary structure of the Esp protein from strain MMH594, inferred to be 1,873 amino acids (aa) with a predicted mass of similar to 202 kDa, reveals a core region consisting of repeat unit s that make up 50% of the protein. Esp bears global organizational similari ty to the Rib and C alpha proteins of group B streptococci. Identity among Esp, Rib, and C alpha proteins is strikingly localized to a stretch of 13 a a within repeats of similar length. The high degree of conservation of this 13-residue sequence suggests that it plays an important role in the natura l selection for this trait among infection-derived E, faecalis and group B streptococcal isolates.