Aberrations of the tissue factor pathway in patients positive for lupus anticoagulant

Citation
Y. Wakita et al., Aberrations of the tissue factor pathway in patients positive for lupus anticoagulant, CL APPL T-H, 5(1), 1999, pp. 10-15
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology
Journal title
CLINICAL AND APPLIED THROMBOSIS-HEMOSTASIS
ISSN journal
1076-0296 → ACNP
Volume
5
Issue
1
Year of publication
1999
Pages
10 - 15
Database
ISI
SICI code
1076-0296(199901)5:1<10:AOTTFP>2.0.ZU;2-V
Abstract
To evaluate the relationship between the tissue factor (TF) pathway and lup us anticoagulant (LA), in the present study, we measured the plasma levels of TF antigen and TF pathway inhibitor (TFPI) antigen in patients positive for LA. Plasma TF and TFPI levels in LA-positive patients were significantl y higher than levels in healthy volunteers (p < 0.01). In LA-positive patie nts, there were no significant differences in plasma TF and TFPI levels bet ween patients with and without thrombosis. In patients with thrombosis, the re was no significant difference in the plasma TF level between LA-positive and LA-negative patients; however, the plasma TFPI level in LA-positive pa tients was significantly lower than that in LA-negative patients (p < 0.01) . We also examined the TF pathway in human umbilical venous endothelial cel ls (HUVEC) incubated with plasma of LA-positive patients, LA-negative patie nts, and healthy volunteers. TF activity was significantly higher (p <.05) in HUVECs incubated with the plasma of LA-positive patients than in cells i ncubated with the plasma of the other two groups (p <.01). However, there w as no significant difference in TFPI antigen levels among the media of HUVE Cs incubated with the plasma of all groups. The viability of HUVEC incubate d with the plasma of LA-positive patients with thromboses, LA-positive pati ents without thromboses, and LA-negative patients with thromboses were sign ificantly lower than that of HUVECs incubated with the plasma of healthy vo lunteers (p <.01). These findings suggest that abnormalities of the TF path way plays an important role in the mechanism of hypercoagulability in LA-po sitive patients. LA may affect vascular endothelial cells causing thromboge nesis.