Diffusion of transferrin receptor clusters

Citation
M. Srivastava et No. Petersen, Diffusion of transferrin receptor clusters, BIOPHYS CH, 75(3), 1998, pp. 201-211
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics","Physical Chemistry/Chemical Physics
Journal title
BIOPHYSICAL CHEMISTRY
ISSN journal
0301-4622 → ACNP
Volume
75
Issue
3
Year of publication
1998
Pages
201 - 211
Database
ISI
SICI code
0301-4622(199812)75:3<201:DOTRC>2.0.ZU;2-I
Abstract
Two dimensional motion of membrane receptors provides a mechanism for inter action among receptors in the plane of the membrane. In some cases the late ral diffusion leads to formation of clusters which may also be mobile. We h ave used image cross-correlation (ICCS) spectroscopy technique to measure t he translational motion of transferrin receptors in the membrane of 3T3 fib roblasts and HEp2 carcinoma cells. The technique is based on the measuremen t and analysis of fluctuations in the intensity observed in fluorescence co nfocal microscope images measured as a function of time. The fluorescence f luctuations arise from stochastic concentration fluctuations about the equi librium concentration caused by movement of receptors. The amplitude of the fluctuations depend on the number of fluorescent molecules in the observat ion volume and the dynamics provide the rate of movement. The diffusion obs erved by this analysis is orders of magnitude slower than that measured by conventional photobleaching techniques. The slower motion corresponds to th e diffusion of receptor clusters which provide the more dominant fluctuatio ns. (C) 1998 Elsevier Science B.V. All rights reserved.