Homogeneity of 16S-23S ribosomal intergenic spacer regions of Tropheryma whippelii in Swiss patients with Whipple's disease

Citation
Hp. Hinrikson et al., Homogeneity of 16S-23S ribosomal intergenic spacer regions of Tropheryma whippelii in Swiss patients with Whipple's disease, J CLIN MICR, 37(1), 1999, pp. 152-156
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
0095-1137 → ACNP
Volume
37
Issue
1
Year of publication
1999
Pages
152 - 156
Database
ISI
SICI code
0095-1137(199901)37:1<152:HO1RIS>2.0.ZU;2-G
Abstract
The current genetic strategies used to identify Tropheryma whippelii, the p utative agent of Whipple's disease, are based on PCR-mediated amplification of a part of its 16S rRNA gene (16S rDNA). Because there is very little in traspecies variation in these molecules, they are not suitable as targets f or epidemiologic investigations. However, the intergenic spacer region betw een the 16S and 23S rDNAs is usually much more variable and has repeatedly been used for epidemiologic purposes. We have therefore amplified the space r region of T, whippelii directly from clinical specimens from nine indepen dent Swiss patients with Whipple's disease by PCR with primers complementar y to the 3' and 5' ends of the 16S and 23S rDNAs, respectively. The amplico ns were directly sequenced and the sequences were compared to the T. whippe lii reference sequence in GenBank/EMBL (accession no. X99636). Complete seq uence homogeneity was found between the samples from our nine patients; the spacer sequence was also identical to the reference sequence. However, the sequences corresponding to the 3' and 5' ends of the 16S and the 23S rDNAs of T. whippelii, respectively, differed from the respective sequences in G enBank/EMBL. The same sequence found in our patients was then found in a sa mple from the German patient from which the published sequence had been der ived. We conclude that the 16S-23S rDNA spacer region seems to be very cons erved in T. whippelii and that the respective reference entry in public dat abases should be revised.