Glutathione downregulates the phosphorylation of I kappa B: Autoloop regulation of the NF-kappa B-mediated expression of NF-kappa B subunits by TNF-alpha in mouse vascular endothelial cells

Citation
S. Cho et al., Glutathione downregulates the phosphorylation of I kappa B: Autoloop regulation of the NF-kappa B-mediated expression of NF-kappa B subunits by TNF-alpha in mouse vascular endothelial cells, BIOC BIOP R, 253(1), 1998, pp. 104-108
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
253
Issue
1
Year of publication
1998
Pages
104 - 108
Database
ISI
SICI code
0006-291X(199812)253:1<104:GDTPOI>2.0.ZU;2-O
Abstract
Nuclear factor-kappa B (NF-kappa B) regulates gene expression upon immune a nd inflammatory responses. It has been demonstrated that redox regulation b y thiols is involved in the signal-transduction cascade. In this study, we examined the effect of glutathione (GSH) on the NF-kappa B activity and the expression of NF-kappa B subunits induced by tumor necrosis factor-alpha ( TNF-alpha) using mouse vascular endothelial cells. GSH inhibited the serine phosphorylation of I kappa B-alpha by TNF-alpha, leading to the downregula tion of NF-kappa B-DNA binding activity followed by decreased expression of p65/p50 and I kappa B mRNAs. The regulation of the autoregulatory loop for the NF-kappa B activation and the expression of NF-kappa B subunits may be important in endothelial cells in response to cytokines. (C) 1998 Academic Press.