Murine cytomegalovirus induces a Sjogren's syndrome-like disease in C57B1/6-lpr/lpr mice

Citation
M. Fleck et al., Murine cytomegalovirus induces a Sjogren's syndrome-like disease in C57B1/6-lpr/lpr mice, ARTH RHEUM, 41(12), 1998, pp. 2175-2184
Citations number
51
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Rheumatology,"da verificare
Journal title
ARTHRITIS AND RHEUMATISM
ISSN journal
0004-3591 → ACNP
Volume
41
Issue
12
Year of publication
1998
Pages
2175 - 2184
Database
ISI
SICI code
0004-3591(199812)41:12<2175:MCIASS>2.0.ZU;2-3
Abstract
Objective. To analyze Fas and tumor necrosis factor receptor I (TNFRI) apop tosis pathways in salivary gland inflammatory disease induced by murine cyt omegalovirus (MCMV) infection. Methods. Four different strains of mice (C57Bl/6 [B6]-+/+, Fas-deficient B6 -lpr/lpr, TNFRI-deficient B6-tnfr1(0/0), and B6-tnfr1(0/0)-lpr/lpr mice) we re infected intraperitoneally with the Smith strain of MCMV (1 x 10(5) plaq ue-forming units). Viral load was determined by a plaque assay, inflammatio n and apoptosis by immunohistochemistry and staining with terminal dUTP nic k-end labeling, and autoantibodies by enzyme-linked immunosorbent assay. Results. Infectious MCMV was not detectable by day 100, Although all MCMV-i nfected mice developed acute sialadenitis by day 28, a chronic (>100 days), severe salivary gland inflammation and anti-Re and anti-La antibodies deve loped only in the B6-lpr/lpr mice. Apoptotic cells were detected during the acute, but not the chronic, phase of inflammation. Conclusion. Both Fas- and TNFRI-mediated apoptosis contribute to the cleara nce of MCMV-infected cells in the salivary glands. However, because Fas-med iated apoptosis is necessary for the downmodulation of the immune response, a defect in this process can lead to a postinfection, chronic inflammatory response that resembles Sjogren's syndrome.