Collagen type I modulates the platelet-derived growth factor (PDGF) regulation of the growth and expression of beta 1 integrins by rat mesangial cells

Citation
S. Kagami et al., Collagen type I modulates the platelet-derived growth factor (PDGF) regulation of the growth and expression of beta 1 integrins by rat mesangial cells, BIOC BIOP R, 252(3), 1998, pp. 728-732
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
252
Issue
3
Year of publication
1998
Pages
728 - 732
Database
ISI
SICI code
0006-291X(19981127)252:3<728:CTIMTP>2.0.ZU;2-J
Abstract
Mesangial cell (RLC) proliferation and the deposition of collagen type I (c ollagen I) are the major pathological features in many types of glomerulone phritis (GN). Recent work suggested that beta-integrins play a critical rol e in the cell proliferation and extracellular matrix (ECM) remodeling obser ved in tissue repair after injury. To examine the involvement of beta-integ rins in MC proliferation in association with the interaction of MCs with pa thological collagen I, we investigated the effect of a prominent mitogen, p latelet-derived growth factor-BE (PDGF-EE) on the growth and expression of beta-integrins by MCs cultured on plastic or in a three-dimensional collage n I gel. Immunoprecipitation using S-35-metabolic labeling, flow cytometry and a H-3-thymidine-uptake analysis demonstrated that PDGF-BB stimulated th e cell mitogenicity and the expression of alpha 5 beta 1 integrin (a fibron ectin receptor), but not alpha 1 beta 1 integrin (a collagen and laminin re ceptor) of MCs on plastic, in a dose-dependent manner. In contrast, MCs in the collagen I gels showed no significant changes in mitogenicity or alpha 1 beta 1 and alpha 5 beta 1 integrin expression, but increased alpha 1 beta 1 integrin-mediated gel contraction was observed after PDGF-BE stimulation . Thus, the parallel up-regulation of MC-mitogenicity and alpha 5 beta 1 in tegrin expression by PDGF-BB suggested that alpha 5 beta 1 integrin is an i mportant ECM receptor involved in the proliferative phenotype of MC. A spat ial interaction between MCs and pathological collagen I in GN may influence the PDGF regulation of the MC phenotype regarding the cell growth and the expression of beta 1 integrins. (C) 1998 Academic Press.