W. Blumenfeld et al., Comparison of aspiration, touch and scrape preparations simultaneously obtained from surgically excised specimens - Effect of different methods of smear preparation on interpretive cytologic features, ACT CYTOL, 42(6), 1998, pp. 1414-1418
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
OBJECTIVE: To determine whether three commonly used methods of obtaining an
d preparing cells for cytologic evaluation-touch preparation (TP),fine need
le aspiration (FNA) and scrape preparation-yield essentially comparable mat
erial for evaluation or whether important differences exist between them th
at may influence interpretation.
STUDY DESIGN: FNA, TP and scrape preparations were made simultaneously on s
urgically excised specimens. Thirty breast, 6 lung and 12 colon lesions wer
e studied. Each slide was assessed for qualitative and quantitative feature
s. Cellularity was semiquantitatively scored.
RESULTS: Scrape preparations were the most cellular (P < .0001). Scrape and
TPs often had larger tissue fragments than FNA. However, fragmentation of
epithelial groups into smaller clusters and single cells was often noted or
? scrape preparations FNAs tended to have definer backgrounds than the othe
r two methods.
CONCLUSION: Most often, there are few differences between smears prepared b
y the three techniques studied. However, scrape preparations may yield smea
rs with smaller clusters and single cells as compared to the other two meth
ods; that is a potential source of diagnostic confusion, particularly with
respect to benign breast lesions. Scrape preparations uniformly yield more
cellular smears. To the extent that cellularity is an interpretive factor i
n assessing cytologic specimens, it is important to be aware of the increas
ed cellularity of scrape preparations as compared to the other two techniqu