Comparison of aspiration, touch and scrape preparations simultaneously obtained from surgically excised specimens - Effect of different methods of smear preparation on interpretive cytologic features

Citation
W. Blumenfeld et al., Comparison of aspiration, touch and scrape preparations simultaneously obtained from surgically excised specimens - Effect of different methods of smear preparation on interpretive cytologic features, ACT CYTOL, 42(6), 1998, pp. 1414-1418
Citations number
9
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Journal title
ACTA CYTOLOGICA
ISSN journal
0001-5547 → ACNP
Volume
42
Issue
6
Year of publication
1998
Pages
1414 - 1418
Database
ISI
SICI code
0001-5547(199811/12)42:6<1414:COATAS>2.0.ZU;2-5
Abstract
OBJECTIVE: To determine whether three commonly used methods of obtaining an d preparing cells for cytologic evaluation-touch preparation (TP),fine need le aspiration (FNA) and scrape preparation-yield essentially comparable mat erial for evaluation or whether important differences exist between them th at may influence interpretation. STUDY DESIGN: FNA, TP and scrape preparations were made simultaneously on s urgically excised specimens. Thirty breast, 6 lung and 12 colon lesions wer e studied. Each slide was assessed for qualitative and quantitative feature s. Cellularity was semiquantitatively scored. RESULTS: Scrape preparations were the most cellular (P < .0001). Scrape and TPs often had larger tissue fragments than FNA. However, fragmentation of epithelial groups into smaller clusters and single cells was often noted or ? scrape preparations FNAs tended to have definer backgrounds than the othe r two methods. CONCLUSION: Most often, there are few differences between smears prepared b y the three techniques studied. However, scrape preparations may yield smea rs with smaller clusters and single cells as compared to the other two meth ods; that is a potential source of diagnostic confusion, particularly with respect to benign breast lesions. Scrape preparations uniformly yield more cellular smears. To the extent that cellularity is an interpretive factor i n assessing cytologic specimens, it is important to be aware of the increas ed cellularity of scrape preparations as compared to the other two techniqu es.