CHARACTERIZATION OF CIRCULATING ERYTHROCYTES FROM MYELODYSPLASTIC PATIENTS TREATED WITH RECOMBINANT-HUMAN-ERYTHROPOIETIN

Citation
S. Shapiro et al., CHARACTERIZATION OF CIRCULATING ERYTHROCYTES FROM MYELODYSPLASTIC PATIENTS TREATED WITH RECOMBINANT-HUMAN-ERYTHROPOIETIN, Leukemia, 7(9), 1993, pp. 1328-1333
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology,Oncology
Journal title
ISSN journal
0887-6924
Volume
7
Issue
9
Year of publication
1993
Pages
1328 - 1333
Database
ISI
SICI code
0887-6924(1993)7:9<1328:COCEFM>2.0.ZU;2-8
Abstract
Age-density fractionation, in-vitro erythrophagocytosis, and enumerati on of membrane-bound antibodies were monitored for circulating red blo od cells (RBC) from five anemic patients with myelodysplastic syndrome s (MDS), in relation to administration of recombinant human erythropoi etin (rhEPO). The density distribution patterns of erythrocytes from t he patients prior to treatment were in accordance with their inability to produce compensating levels of circulating RBC. The complete respo nse of one patient to rhEPO and partial responses of two other patient s were accompanied by shifts to larger proportions of low density (you ng) RBC. In vitro phagocytosis of density-fractionated RBC from the co mplete responder was similar to those of age-matched non-anemic donors . Elevated erythrophagocytosis prior to rhEPO administration was obser ved for the partial responders and further increased during treatment in one, suggesting the stimulation of abnormal progenitors producing h ighly defective erythrocytes. There was no correlation between levels of erythrophagocytosis and RBC membrane-bound immunoglobulins in this group of patients. Our findings suggest that density distribution anal ysis of circulating RBC coupled with in vitro erythrophagocytosis may provide useful predictive tools for selecting potential responders to rhEPO administration among anemic MDS patients.