RUBISCO BUT NOT RUBISCO ACTIVASE IS CLUSTERED IN THE CARBOXYSOMES OF THE CYANOBACTERIUM SYNECHOCOCCUS SP PCC-7942 - MUD-INDUCED CARBOXYSOMELESS MUTANTS

Citation
D. Friedberg et al., RUBISCO BUT NOT RUBISCO ACTIVASE IS CLUSTERED IN THE CARBOXYSOMES OF THE CYANOBACTERIUM SYNECHOCOCCUS SP PCC-7942 - MUD-INDUCED CARBOXYSOMELESS MUTANTS, Molecular microbiology, 9(6), 1993, pp. 1193-1201
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
ISSN journal
0950-382X
Volume
9
Issue
6
Year of publication
1993
Pages
1193 - 1201
Database
ISI
SICI code
0950-382X(1993)9:6<1193:RBNRAI>2.0.ZU;2-R
Abstract
The Mud technology of Groisman and Casadaban was adapted to the cyanob acterium Synechococcus sp. PCC 7942. A new high-CO2-requiring (hcr) mu tant, hcr Mu28 was isolated following the integration of the Mud eleme nt 89 bp upstream of ORFI, at the 5'-flanking region of the rbc operon , which encodes RuBP carboxylase/oxygenase (Rubisco). The integration involved a 7 bp duplication that formed a direct repeat at the integra tion site, as previously shown in Escherichia coli. The mutant was dev oid of apparent carboxysome bodies, which are considered to be importa nt for the availability of CO2 for Rubisco. Immunolabelling studies de monstrated that Rubisco was distributed throughout hcr Mu28 cells, whi le in the wild type (WT) and in the carboxysome aberrant mutant hcr O2 21, Rubisco was markedly associated with the carboxysomes. Rubisco act ivase, however, was evenly distributed throughout the cytosol of the h cr and WT cells, without any preferential association with the apparen t carboxysomes.