DIABETIC STATE-MODIFIED MACROPHAGES IN GK RAT RELEASE PLATELET-DERIVED GROWTH FACTOR-BB FOR TUBE FORMATION OF ENDOTHELIAL-CELLS IN RAT AORTA

Citation
S. Kobayashi et al., DIABETIC STATE-MODIFIED MACROPHAGES IN GK RAT RELEASE PLATELET-DERIVED GROWTH FACTOR-BB FOR TUBE FORMATION OF ENDOTHELIAL-CELLS IN RAT AORTA, Immunopharmacology, 35(2), 1996, pp. 171-180
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Pharmacy",Immunology
Journal title
ISSN journal
0162-3109
Volume
35
Issue
2
Year of publication
1996
Pages
171 - 180
Database
ISI
SICI code
0162-3109(1996)35:2<171:DSMIGR>2.0.ZU;2-0
Abstract
Cultured peritoneal macrophages (M0) of GK rats, a non insulin-depende nt diabetes mellitus model established from normal Wistar rats, increa sed the tube formation of aortic endothelial cells (EC). A polyclonal anti-platelet-derived growth factor (PDGF)-BB (0.21-3.3 mu g/ml) inhib ited (by 66.5 +/- 6.6%) the tube-forming activity of conditioned mediu m (CM) from M0 in diabetic GK rats, but not that in age-matched normal Wistar rats. A polyclonal anti-interleukin (IL)-1 alpha (0.16-0.33%) also inhibited (by 37.7 +/- 5.8%) the activity of diabetic M0-CM, and its inhibitory effect was smaller than that of anti-PDGF-BB. A monoclo nal anti-basic fibroblast growth factor (bFGF) (0.6-60 ng/ml) inhibite d the activities of CM from both Mel, respectively. The tube-forming a ctivity of the normal M0-CM was increased by the serum isolated from t he diabetic GK rat more than by that from the age-matched normal Wista r rat. The activity of normal M0-CM was also increased by 16.7-50 mM g lucose-exposed serum. These tube-forming activities of the CM of M0 st imulated by diabetic serum and by the high concentration of glucose-tr eated serum were completely inhibited by anti-PDGF-BB. In conclusion, PDGF-BB was selectively released from the diabetic state-modified M0 i n the GK rat to increase tube formation, suggesting a key role in the cause of angiogenesis. The diabetic state-induced activation may depen d on advanced glycosylation endproducts produced in the serum in the d iabetic rat.