ELECTRON-MICROSCOPIC CYTOCHEMICAL STUDIES ON SULFATED GLYCOSAMINOGLYCANS IN RAT MAST-CELL GRANULES

Authors
Citation
J. Kida, ELECTRON-MICROSCOPIC CYTOCHEMICAL STUDIES ON SULFATED GLYCOSAMINOGLYCANS IN RAT MAST-CELL GRANULES, Acta histochemica et cytochemica, 26(2), 1993, pp. 135-146
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cytology & Histology
ISSN journal
0044-5991
Volume
26
Issue
2
Year of publication
1993
Pages
135 - 146
Database
ISI
SICI code
0044-5991(1993)26:2<135:ECSOSG>2.0.ZU;2-T
Abstract
Electron microscopic cytochemical studies have been made on sulfated g lycosaminoglycans in mast cell granules from the rat peritoneal cavity by means of combined alcian blue (AB) pH 1.0-phosphotungstic acid (PT A) staining and enzyme (heparinase or chondroitinase ABC) digestion or chemical modification (nitrous acid) procedures. The results obtained revealed that the mast cell populations from the rat peritoneal cavit y are grouped into at least three cell types on the basis of the molec ular species of sulfated glycosaminoglycans contained in the cytoplasm ic granules, the first cell type occupying the majority of the cell po pulation with cytoplasmic granules containing primarily heparin, the s econd cell type being of an exceedingly small proportion with cytoplas mic granules containing primarily isomeric chondroitin sulfate (D and/ or E), and the third cell type (intermediates between the first and se cond types) constituting a relatively small proportion with cytoplasmi c granules containing two kinds of cytoplasmic granules containing hep arin and isomeric chondroitin sulfate (D and/or E) respectively. In ad dition, unique substructures of cytoplasmic granules containing differ ent sulfated glycosaminoglycans were disclosed such as tubular or thin thread-like elements, interstitial vesicles and surface spine-like fi gures. The present results are taken to represent the first electron m icroscopic cytochemical evidence that mast cell populations are hetero geneous in terms of molecular species of sulfated glycosaminoglycans c ontained in the cytoplasmic granules in mammals.