CELL-BIOMATERIAL INTERACTIONS - ROLE OF TRANSGLUTAMINASE ENZYME

Citation
G. Peluso et al., CELL-BIOMATERIAL INTERACTIONS - ROLE OF TRANSGLUTAMINASE ENZYME, Journal of materials science. Materials in medicine, 7(12), 1996, pp. 707-711
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Polymer Sciences","Medicine Miscellaneus","Materials Science, Biomaterials
ISSN journal
0957-4530
Volume
7
Issue
12
Year of publication
1996
Pages
707 - 711
Database
ISI
SICI code
0957-4530(1996)7:12<707:CI-ROT>2.0.ZU;2-2
Abstract
Cell interaction(s) with biomaterial undergo a multistep-step paradigm of adhesion, contact, attachment, and spreading. However, the molecul ar mechanism(s) underlying the cell anchorage on a material surface is not yet well understood. Transglutaminases, a family of calcium-depen dent enzymes, has been implicated in the interactions between cells an d extracellular matrix. To investigate the functions of this enzyme in cell-material interactions, Balb-C 3T3 fibroblasts were cultured, sta bly expressing high levels of tissue transglutaminase (TGase), on dish es precoated with thin layers of the hydrophilic hydrogel poly (2-hydr oxyethyl methacrylate) (polyHEMA). Results suggest that in the presenc e of a relatively high poly (HEMA) dilution, the transfected cells sho wed a more complex morphologic pattern, characterized by many spread a nd flattened cells, in comparison with a control culture. These studie s support the hypothesis that the overexpression of tissue transglutam inase may contribute both to changes in cellular morphology and adhesi veness.