EVALUATION OF A NEW MEASUREMENT PROCEDURE FOR THE CONCENTRATION OF FERRITIN IN SERUM

Citation
T. Palenciadominguez et al., EVALUATION OF A NEW MEASUREMENT PROCEDURE FOR THE CONCENTRATION OF FERRITIN IN SERUM, European journal of clinical chemistry and clinical biochemistry, 35(2), 1997, pp. 117-120
Citations number
8
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Medical Laboratory Technology
ISSN journal
0939-4974
Volume
35
Issue
2
Year of publication
1997
Pages
117 - 120
Database
ISI
SICI code
0939-4974(1997)35:2<117:EOANMP>2.0.ZU;2-M
Abstract
The aim of this study was the evaluation of an immunoturbidimetric mea surement procedure for the concentration of ferritin in serum, based o n the new Tina-quant Ferritin reagents kit (Boehringer Mannheim) in wh ich the antibody-coated latex particles have been modified with regard to the previous Tina-quant Ferritin reagents kit. The evaluation was carried out using the BM/Hitachi 917 Analyzer. The evaluation included the within-run and between-day imprecisions estimation, the compariso n of measurement procedures and the assessment of the measuring range. An additional study of between-day imprecision was done using 4 mu l and 20 mu l of sample. Detection limit was studied using BM/Hitachi 91 7 (4 mu l and 20 mu l of sample), ES 300 and Cobas-Core analyzers. The coefficients of variation obtained in the between-day imprecision stu dy are lower when 20 mu l of sample is used instead of 4 mu l. In the measurement procedure using 20 mu l, the coefficient of variation obse rved at physiological concentrations is 2.4%. The detection limit with 20 mu l of sample is lower than with 4 mu l. Therefore, in ferropenic anaemia studies it is convenient to use the measurement procedure wit h 20 mu l sample volumes, since it possesses the best metrological cha racteristics for this purpose.