MOLECULAR-CLONING AND LOCALIZATION OF RHODOPSIN KINASE IN THE MAMMALIAN PINEAL

Citation
Xy. Zhao et al., MOLECULAR-CLONING AND LOCALIZATION OF RHODOPSIN KINASE IN THE MAMMALIAN PINEAL, Visual neuroscience, 14(2), 1997, pp. 225-232
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0952-5238
Volume
14
Issue
2
Year of publication
1997
Pages
225 - 232
Database
ISI
SICI code
0952-5238(1997)14:2<225:MALORK>2.0.ZU;2-E
Abstract
Several retinal photoreceptor proteins involved in phototransduction h ave also been found in the mammalian pineal. This study demonstrates t hat rat and human pineals express protein kinases that are identical t o the corresponding rod photoreceptor rhodopsin kinases. The deduced a mino acid sequence of rat and human rhodopsin kinases have 84% sequenc e similarity to the earlier reported sequence of the bovine retinal en zyme, with complete conservation of the topological regions containing the position of the catalytic domain and sites of posttranslational m odifications. Rat pineal also expresses rod opsin and putative blue co ne opsin. Using immunocytochemistry, rod opsin and rhodopsin kinase we re found to be co-localized in pinealocytes in the human tissue. These data demonstrate that the mammalian pineal contains light-sensitive o psins and a kinase involved in their inactivation. These findings corr elate with an earlier report that neonatal rats show extraretinal ligh t sensitivity, and suggest that a functional photoreceptive system may be present in the adult mammalian pineal.