UV-RADIATION PROTECTING EFFICACY OF THIOLS, STUDIED WITH UVA-INDUCED BINDING OF 8-MOP AND CPZ TO RAT EPIDERMAL BIOMACROMOLECULES INVIVO

Citation
Lt. Vandenbroeke et Gmj. Vanhenegouwen, UV-RADIATION PROTECTING EFFICACY OF THIOLS, STUDIED WITH UVA-INDUCED BINDING OF 8-MOP AND CPZ TO RAT EPIDERMAL BIOMACROMOLECULES INVIVO, International journal of radiation biology & related studies in physics, chemistry & medicine, 63(4), 1993, pp. 493-500
Citations number
63
Language
INGLESE
art.tipo
Article
ISSN journal
0020-7616
Volume
63
Issue
4
Year of publication
1993
Pages
493 - 500
Database
ISI
SICI code
0020-7616(1993)63:4<493:UPEOTS>2.0.ZU;2-P
Abstract
The following topically-applied thiols were investigated with regard t o their possible UV-radiation protective properties: captopril, cystea mine, ergothioneine, mesna, mercaptopropionylglycine, N-acetyl-cystein e and penicillamine. As a measure for protection the inhibition of in vivo irreversible photobinding of the labelled phototoxic drugs chlorp romazine (CPZ) and 8-methoxypsoralen (8-MOP) to rat epidermal biomacro molecules was used. Ergothioneine, mesna and penicillamine did not sho w any effect; probably, as a result of their charge they are not able to enter the stratum corneum. Captopril, cysteamine, mercaptopropionyl glycine and N-acetylcysteine showed a considerable inhibition of CPZ a nd 8-MOP photobinding. Captopril and N-acetylcysteine were clearly the most potent whereas cysteamine was the least effective. Captopril, me rcaptopropionylglycine and N-acetylcysteine appeared to have a wider a ction range and to be a more effective protector than dl-alpha-tocophe rol and di-butyl-hydroxytoluene. Cysteamine and mercaptopropionylglyci ne were only capable of protecting the stratum corneum. Captopril and N-acetylcysteine on the other hand showed an additional dose-dependent inhibition of photobinding to the viable epidermis. Gradually with in creasing time after application, the protecting efficacy with regard t o the viable layer of the epidermis decreased; the duration of protect ion depending on the dose.