Rg. Bogle et al., IDENTIFICATION OF INHIBITORS OF NITRIC-OXIDE SYNTHASE THAT DO NOT INTERACT WITH THE ENDOTHELIAL-CELL L-ARGININE TRANSPORTER, British Journal of Pharmacology, 105(4), 1992, pp. 768-770
The effects of inhibitors of nitric oxide (NO) synthase and other cati
onic amino acids on unidirectional L-arginine transport were studied i
n porcine aortic endothelial cells cultured in microwell plates or per
fused in microcarrier columns. L-Homoarginine, L-lysine and L-ornithin
e inhibited transport of L-arginine. The NO synthase inhibitors N(G)-m
onomethyl-L-arginine and N(G)-iminoethyl-L-ornithine also reduced L-ar
ginine uptake, whereas N(G)-nitro-L-arginine and its methyl-ester had
no inhibitory effect. The ability to modulate selectively endothelial
cell L-arginine transport or NO synthase activity will allow further c
haracterization of the arginine transporter and its role in regulating
NO biosynthesis.