EXPRESSION AND GROWTH-PROMOTING EFFECT OF ADULT T-CELL LEUKEMIA-DERIVED FACTOR - A HUMAN THIOREDOXIN HOMOLOG IN HEPATOCELLULAR-CARCINOMA

NAKAMURA H MASUTANI H TAGAYA Y YAMAUCHI A INAMOTO T NANBU Y FUJII S OZAWA K YODOI J

H. Nakamura et al., EXPRESSION AND GROWTH-PROMOTING EFFECT OF ADULT T-CELL LEUKEMIA-DERIVED FACTOR - A HUMAN THIOREDOXIN HOMOLOG IN HEPATOCELLULAR-CARCINOMA, Cancer, 69(8), 1992, pp. 2091-2097

28

INGLESE

Article

Cancer → ACNP

0008-543X

69

8

1992

2091 - 2097

ISI

0008-543X(1992)69:8<2091:EAGEOA>2.0.ZU;2-I

Adult T-cell leukemia-derived factor (ADF), originally defined as an i nterleukin-2 receptor inducer, is a human thioredoxin homologue. ADF i s detected in many malignant tissues and has a growth-promoting effect on transformed cells. In this study, ADF expression was examined immu nohistochemically in human liver cell lines and liver tissues, and its growth-promoting effect was tested on human hepatoma cells. on three liver cell lines -PLC/PRF/5, HepG2, and Chang liver cells-ADF stained positively and also was detected by immunoblotting. ADF had strong sta ining in the fetal liver (n = 8), although it was faint in the normal adult liver (n = 6). In hepatocellular carcinoma (n = 25), ADF express ion generally was enhanced and was very strong in 52% (13 of 25) of th e cases, although it was moderate in cases of chronic hepatitis or cir rhosis. ADF augmented the growth of PLC/PRF/5 cells and showed an addi tive effect with epidermal growth factor. These results indicate possi ble involvement of ADF in cell activation and growth of hepatocytes, a s is the case with lymphocytes.