ESTABLISHMENT AND CHARACTERIZATION OF A PANEL OF HUMAN LUNG-CANCER CELL-LINES

CAMPLING BG HAWORTH AC BAKER HM GREER DL HOLDEN JJA BRADLEY WEC PYM J DEXTER DF

Bg. Campling et al., ESTABLISHMENT AND CHARACTERIZATION OF A PANEL OF HUMAN LUNG-CANCER CELL-LINES, Cancer, 69(8), 1992, pp. 2064-2074

53

INGLESE

Article

Cancer → ACNP

0008-543X

69

8

1992

2064 - 2074

ISI

0008-543X(1992)69:8<2064:EACOAP>2.0.ZU;2-4

The establishment and characterization of 11 human lung cancer cell li nes are described in this article. Nine of these cell lines were estab lished over a 5-year period, from 1983 to 1988, from patients treated at the Kingston Regional Cancer Centre. These include eight definite o r probable small cell lung cancer (SCLC) lines and one adenocarcinoma line. In addition, two other SCLC cell lines were characterized. All o f the lines have been in continuous culture for more than 2 years. The clinical histories of the patients from whom the cell lines were deri ved are outlined here. Several features of the cell lines are presente d, including the following: (1) a comparison of the histologic feature s of the cell lines with the original biopsy specimens; (2) the expres sion of various markers, including cytokeratin, carcinoembryonic antig en, calcitonin, and neuron-specific enolase; (3) activities of the enz ymes 1-dopa decarboxylase and the brain isoenzyme of creatine kinase; (4) growth characteristics; (5) cloning efficiency in soft agar; (6) t umorigenicity in nude mice; and (7) cytogenetic studies. These cell li nes, obtained directly from patients with a spectrum of drug-sensitive and drug-resistant tumors, will be valuable in vitro models of sensit ivity and resistance to chemotherapy in lung cancer.