INHIBITION OF PENTA-ACETYL GENIPOSIDE ON AFB1-INDUCED GENOTOXICITY INC3H10T(1 2) CELLS/

Citation
Th. Tseng et al., INHIBITION OF PENTA-ACETYL GENIPOSIDE ON AFB1-INDUCED GENOTOXICITY INC3H10T(1 2) CELLS/, Cancer letters, 62(3), 1992, pp. 233-242
Citations number
24
Language
INGLESE
art.tipo
Article
Journal title
ISSN journal
0304-3835
Volume
62
Issue
3
Year of publication
1992
Pages
233 - 242
Database
ISI
SICI code
0304-3835(1992)62:3<233:IOPGOA>2.0.ZU;2-C
Abstract
A new compound, penta-acetyl geniposide ((Ac)5-GP), was obtained from modified extract of Gardenia fructus (San-Jee-Chee in Chinese). The st ructure of the compound was identified as -2',3',4',6'-tetraacetylgluc opyrannosyloxyl)-1,4a, hydro-7-(acetomethyl)-cyclopentapyran-4-carboxy lic acid methyl ester, according to the spectral data. The inhibitory effects of (Ac)5-GP on aflatoxin B1 (AFB1)-induced cytotoxicity and DN A damage were studied. In the investigation of the inhibitory effect o f (Ac)5-GP on AFB1-cytotoxicity, the plating efficiency of C3H10T1/2 c ells in S-9 activation system was increased. In addition, (Ac)5-GP inh ibited the DNA damage of AFB1-treated C3H10T1/2 cells, and it interfer ed with the inhibitory effect of DNA synthesis caused by AFB1. These r esults suggest that the reduced DNA damage and the increased DNA synth esis from cultured C3H10T1/2 cells are important mechanisms for the in hibition of AFB1-cytotoxicity by (Ac)5-GP.