TRANSIENT INDUCTION OF TRYPTOPHAN DECARBOXYLASE (TDC) AND STRICTOSIDINE SYNTHASE (SS) GENES IN CELL-SUSPENSION CULTURES OF CATHARANTHUS-ROSEUS

Citation
Ia. Roewer et al., TRANSIENT INDUCTION OF TRYPTOPHAN DECARBOXYLASE (TDC) AND STRICTOSIDINE SYNTHASE (SS) GENES IN CELL-SUSPENSION CULTURES OF CATHARANTHUS-ROSEUS, Plant cell reports, 11(2), 1992, pp. 86-89
Citations number
18
Language
INGLESE
art.tipo
Article
Journal title
ISSN journal
0721-7714
Volume
11
Issue
2
Year of publication
1992
Pages
86 - 89
Database
ISI
SICI code
0721-7714(1992)11:2<86:TIOTD(>2.0.ZU;2-J
Abstract
When cell suspension cultures of Catharanthus roseus are treated with autoclaved elicitor from the fungus Pythium aphanidermatum, they respo nd with the rapid transient induction of tryptophan decarboxylase (TDC ) and strictosidine synthase (SS) enzyme activities, followed by the a ccumulation of indole alkaloids (Eilert et al., 1987). In this report, we demonstrate that expression of TDC and SS enzyme activities is pre ceeded by the transient appearance of mRNAs for both enzymes, suggesti ng transcriptional control of these events. The strong transient accum ulation of both TDC and SS enzyme transcripts observed in elicitor-tre ated cell suspension cultures contrasts with the barely detectable lev el of TDC transcripts and the undetectable level of SS transcripts obs erved in developing seedlings.