NUCLEAR SHAPE-ANALYSIS FOR THE ASSESSMENT OF LOCAL INVASION AND METASTASES IN CLINICALLY LOCALIZED PROSTATE CARCINOMA

Citation
Jl. Mohler et al., NUCLEAR SHAPE-ANALYSIS FOR THE ASSESSMENT OF LOCAL INVASION AND METASTASES IN CLINICALLY LOCALIZED PROSTATE CARCINOMA, Cancer, 74(11), 1994, pp. 2996-3001
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology
Journal title
Cancer → ACNP
ISSN journal
0008-543X
Volume
74
Issue
11
Year of publication
1994
Pages
2996 - 3001
Database
ISI
SICI code
0008-543X(1994)74:11<2996:NSFTAO>2.0.ZU;2-5
Abstract
Background. Nuclear shape analysis of histologic sections from radical prostatectomy specimens has retrospectively predicted outcome in pati ents with clinically localized prostate carcinoma. If outcome could be predicted preoperatively by nuclear shape analysis, patients might be selected better for definitive surgical therapy. Morphometric analysi s of preoperative biopsies, however, has not correlated positively wit h values obtained from analysis of prostatectomy specimens. Methods. T he nuclear shapes of histologic specimens of 20 organ-confined carcino mas, 10 periprostatic fat-invasive carcinomas, 10 seminal vesicle-inva sive carcinomas, and 12 lymph node-metastatic carcinomas from 52 patie nts who had undergone radical prostatectomy for clinically localized d isease were evaluated.Results. Nuclei from areas of extraprostatic inv asion or regional lymph node metastases were less round than those fro m the corresponding intraprostatic portion of the tumor (nuclear round ness factor (mean +/- SD) PPF, 51.2 +/- 3.1 vs. 31.2 +/- 3.2; SV, 52.4 +/- 4.1 vs. 31.6 +/- 2.5; and LN, 57.3 +/- 3.1 vs. 36.4 +/- 1.8; pair ed Student's t tests, P < 0.001). Cells sampled from the periphery of organ-confined tumors had a greater nuclear roundness factor (49.1 +/- 1.5) than did those sampled from the center (34.5 +/- 2.0; P < 0.001) or randomly throughout the tumor (37.8 +/- 1.6; P < 0.001). Nuclear r oundness factors for all extraprostatic tumor foci and for peripheral tumor cells in organ-confined disease were similar (analysis of varian ce, P > 0.05). The intraprostatic portions of randomly sampled primary tumors had similar nuclear roundness factors; regardless of pathologi c stage (P > 0.05). Among organ-confined carcinomas, nuclear shape was unrelated to tumor volume. Conclusions. Pathologic stage in clinicall y localized prostate carcinoma cannot be determined by the nuclear sha pe profiles of intraprostatic tumor cells. Thus, patients with a poor prognosis or high pathologic stage can he recognized only when samples for morphometric analysis include high proportions of nuclei from the extraprostatic carcinoma and nuclei from the periphery of organ-confi ned carcinoma that may not be sampled routinely by prostate biopsy.