CYCLIN-A MESSAGE STABILITY VARIES WITH THE CELL-CYCLE

Citation
A. Maity et al., CYCLIN-A MESSAGE STABILITY VARIES WITH THE CELL-CYCLE, Cell growth & differentiation, 8(3), 1997, pp. 311-318
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Cell Biology
ISSN journal
1044-9523
Volume
8
Issue
3
Year of publication
1997
Pages
311 - 318
Database
ISI
SICI code
1044-9523(1997)8:3<311:CMSVWT>2.0.ZU;2-1
Abstract
Progression through the cell cycle in somatic eukaryotic cells is regu lated by variations in the levels of cyclin proteins. These protein le vels are in turn regulated by cyclical oscillations in their mRNA leve ls. We show here that regulation of RNA stability plays a role in the mechanisms underlying cell cycle progression. Both cyclin A and B1 mes sages are expressed at high levels in G(2)-M and at low levels in earl y G(1). The half-lives of their messages mirror this pattern, long in G(2)-M (>8 h) and short in early G(1) (1-2 h). However, there is evide nce of specificity to these changes, because the cyclin A message beco mes stable at the G(1)-S boundary, whereas the cyclin B1 message is un stable until later in S phase. Furthermore, although cyclin B1 mRNA le vels are lowered after irradiation because of enhanced instability, cy clin A mRNA levels and message stability are unaffected by irradiation . Additional evidence of specificity was found in an analysis of cycli n E mRNA stability, which remains constant through the cell cycle, alt hough the cyclin E message displays cell cycle-dependent changes in ex pression. These studies suggest that specific alterations in RNA stabi lity are an important component in regulating the expression of cyclin s A and B1 and hence in controlling the cell cycle.