MOLECULAR-CLONING OF A SOLUBLE FORM OF THE GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR-RECEPTOR ALPHA-CHAIN FROM A MYELOMONOCYTIC CELL-LINE - EXPRESSION, BIOLOGIC ACTIVITY, AND PRELIMINARY-ANALYSIS OF TRANSCRIPT DISTRIBUTION

Citation
Wv. Williams et al., MOLECULAR-CLONING OF A SOLUBLE FORM OF THE GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR-RECEPTOR ALPHA-CHAIN FROM A MYELOMONOCYTIC CELL-LINE - EXPRESSION, BIOLOGIC ACTIVITY, AND PRELIMINARY-ANALYSIS OF TRANSCRIPT DISTRIBUTION, Arthritis and rheumatism, 37(10), 1994, pp. 1468-1478
Citations number
49
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Rheumatology
Journal title
ISSN journal
0004-3591
Volume
37
Issue
10
Year of publication
1994
Pages
1468 - 1478
Database
ISI
SICI code
0004-3591(1994)37:10<1468:MOASFO>2.0.ZU;2-5
Abstract
Objective. To analyze the molecular and functional characteristics of a soluble form of the granulocyte-macrophage colony-stimulating factor receptor alpha chain (sGM-CSFR alpha), and analyze transcript express ion in immune cells and the cellular constituents of rheumatoid arthri tis synovial tissue. Methods. We amplified, cloned, and expressed the sGM-CSFR alpha and transmembrane form of the receptor (tmGM-CSFR alpha ) from complementary DNA derived from a human myelomonocytic cell line . Competitive polymerase chain reaction assays were developed to deter mine the absolute and relative amounts of tmGM-CSFR alpha versus sGM-C SFR alpha message synthesized by various cell lines and tissues. Resul ts. sGM-CSFR alpha transcripts were detected in bone marrow, monocyte/ macrophages (cultured in GM-CSF), rheumatoid synovial tissue, and rheu matoid synovial tissue T cell lines, and represented the predominant t ranscript in synovial fibroblasts and osteoarthritis synovial tissue. Levels of expression in monocyte/macrophages and some synovial fibrobl ast and T cell lines approached those seen in transfected cell lines p roducing functional sGM-CSFR alpha. Conclusion. sGM-CSFR alpha represe nts a functional antagonist of GM-CSF activity in vitro. Expression of sGM-CSFR alpha in bone marrow, rheumatoid synovial tissue T cells, an d synovial fibroblasts suggests an important role in vivo, both in reg ulating myelopoiesis and in modulating the immune response.