EFFECT OF FREEZING ON THE IN-VIVO RECOVERY OF IRRADIATED RED-CELLS

Citation
Cc. Miraglia et al., EFFECT OF FREEZING ON THE IN-VIVO RECOVERY OF IRRADIATED RED-CELLS, Transfusion, 34(9), 1994, pp. 775-778
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology
Journal title
ISSN journal
0041-1132
Volume
34
Issue
9
Year of publication
1994
Pages
775 - 778
Database
ISI
SICI code
0041-1132(1994)34:9<775:EOFOTI>2.0.ZU;2-G
Abstract
Background: Transfusion-associated graft-versus-host disease can be pr evented by gamma radiation of blood components. The increased use of b lood components donated for patients by their family members has resul ted in an increased demand for the storage and handling of irradiated units, and the ability to freeze the cells would allow storage beyond their current expiration date. Study Design and Methods: To assess the effect of freezing and deglycerolization on irradiated red cells, stu dies of autologous radiolabeled red cell recovery were performed using normal volunteers. Each unit of CPDA-1 red cells was immediately divi ded into two equal volumes. Further handling of each half was identica l except that one was irradiated (3500 cGy). The units were grouped un der three protocols: I, irradiated on Day 0 and frozen on Day 5 (n = 4 ); II, irradiated on Day 7, rejuvenated, and frozen on Day 14 (n = 5); and III, irradiated on Day 14, rejuvenated, and frozen on Day 18 (n = 3). All cells were frozen for 3 to 10 months at -80 degrees C. Result s: Irradiated and control units showed no significant differences in s upernatant potassium or hemoglobin. Autologous 24-hour posttransfusion recoveries (mean +/- SD) for the three groups were: I, 89.7 +/- 5.6 p ercent (control, 90.6 +/- 3.2%); II, 85.3 +/- 5.7 percent (control, 83 .7 +/- 3.0%); and III, 79.5 +/- 1.4 percent (control, 82.6 +/- 5.2%). Conclusion: Irradiated red cells can be frozen after being stored unde r various conditions and can still meet established guidelines requiri ng 75-percent recovery 24 hours after transfusion.