THE BIOLOGICAL RESPONSES OF AXOTOMIZED ADULT MOTONEURONS TO BRAIN-DERIVED NEUROTROPHIC FACTOR

Citation
Q. Yan et al., THE BIOLOGICAL RESPONSES OF AXOTOMIZED ADULT MOTONEURONS TO BRAIN-DERIVED NEUROTROPHIC FACTOR, The Journal of neuroscience, 14(9), 1994, pp. 5281-5291
Citations number
64
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0270-6474
Volume
14
Issue
9
Year of publication
1994
Pages
5281 - 5291
Database
ISI
SICI code
0270-6474(1994)14:9<5281:TBROAA>2.0.ZU;2-A
Abstract
Recent studies showed that brain-derived neurotrophic factor (BDNF) pr events developing motoneurons from naturally occurring and axotomy-ind uced cell death. Here we examined whether adult motoneurons retain res ponsiveness to BDNF. Consistent with previous studies, we found that a dult spinal and brainstem motoneurons expressed the mRNA of BDNF recep tor, trkB. in addition, the trkB immunoreactivities were readily detec ted in the adult spinal and brainstem motoneurons. We then demonstrate d that axotomized adult motoneurons responded to exogenous BDNF. BDNF administered locally markedly attenuated the lesion-induced decrease o f ChAT immunoreactivity and activity and enhanced the lesion-induced r eexpression of low-affinity NGF receptor immunoreactivity in adult fac ial motoneurons. Furthermore, we found BDNF administered subcutaneousl y, intravenously, and into the cerebral ventricle attenuated the lesio n-induced decrease of ChAT immunoreactivity in adult facial motoneuron s in a dose-dependent fashion. Our data indicate that adult motoneuron s retain their responsiveness to BDNF, suggesting that BDNF may be use ful as a therapeutic agent for adult motoneuron disease.