A PUTATIVE P-TYPE CU2-TRANSPORTING ATPASE GENE ON CHROMOSOME-II OF SACCHAROMYCES-CEREVISIAE()

Citation
Mr. Rad et al., A PUTATIVE P-TYPE CU2-TRANSPORTING ATPASE GENE ON CHROMOSOME-II OF SACCHAROMYCES-CEREVISIAE(), Yeast, 10(9), 1994, pp. 1217-1225
Citations number
20
Language
INGLESE
art.tipo
Note
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology",Biology
Journal title
YeastACNP
ISSN journal
0749-503X
Volume
10
Issue
9
Year of publication
1994
Pages
1217 - 1225
Database
ISI
SICI code
0749-503X(1994)10:9<1217:APPCAG>2.0.ZU;2-8
Abstract
We have sequenced a gene on the right arm near the telomere of chromos ome II of Saccharomyces cerevisiae which codes for a putative P-type c ation-transporting ATPase (PCA1). The gene codes for a 1216 amino acid s protein. The PCA1 gene expresses a 35 kb message in both haploid and diploid cells when grown in glucose-based rich medium YPD. The gene p roduct is most similar at the C-terminal region to a human copper-tran sporting ATPase and Enterococcus hirae copper-transporting ATPases and also an N-terminal dithiol region that was proposed to be a 'metal-bi nding motif. Cells lacking PCA1 display no obvious phenotype when test ed under standard conditions; whereas they cease growth much earlier t han the isogenic wild-type cells in a minimal medium with high copper concentration. Overexpression of PCA1 under GAL1/10 promoter in yeast cells causes poor growth. We also show that yeast strains carrying PCA 1 in multiple copies grow slower than isogenic wild-type strains in a minimal synthetic medium containing 0.3 mM-CuSO4. The sequence has bee n deposited in the EMBL data library under Accession Number Z29332.