HIGH-MOBILITY-GROUP PROTEIN-1 AND PROTEIN-2 RECOGNIZE CHROMIUM-DAMAGED DNA

Citation
Jf. Wang et al., HIGH-MOBILITY-GROUP PROTEIN-1 AND PROTEIN-2 RECOGNIZE CHROMIUM-DAMAGED DNA, Carcinogenesis, 18(2), 1997, pp. 371-375
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology
Journal title
ISSN journal
0143-3334
Volume
18
Issue
2
Year of publication
1997
Pages
371 - 375
Database
ISI
SICI code
0143-3334(1997)18:2<371:HPAPRC>2.0.ZU;2-L
Abstract
Chromium (Cr) is a human carcinogen and a potent DNA damaging agent, I ncubation of DNA with CrCl3 resulted in dose-dependent binding of Cr t o DNA and, at concentrations >20 mu M, altered the electrophoretic mob ility of a 100 bp oligonucleotide. We also demonstrate that high mobil ity group (HMG) proteins 1 and 2 bind Cr-damaged DNA (Cr-DNA). Protein binding was lesion density-dependent, with maximal binding to DNA tre ated with 100 mu M CrCl3. HMG2 binds to Cr-DNA with a calculated K-d O f similar to 10(-9) M. These proteins also bound DNA obtained from chr omate-treated cells, These results suggest that the covalent attachmen t of Cr to DNA induces alterations in DNA structure which are recogniz ed by HMG1 and HMG2 Therefore, these proteins may function as Cr-damag ed DNA recognition proteins in vivo and as a consequence of binding, m ay play a role in directing the cellular response to Cr-DNA adduct for mation.