A NOVEL EXTRACELLULAR-MATRIX PROTEIN FROM TOMATO ASSOCIATED WITH LIGNIFIED SECONDARY CELL-WALLS

Citation
C. Domingo et al., A NOVEL EXTRACELLULAR-MATRIX PROTEIN FROM TOMATO ASSOCIATED WITH LIGNIFIED SECONDARY CELL-WALLS, The Plant cell, 6(8), 1994, pp. 1035-1047
Citations number
52
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Plant Sciences
Journal title
ISSN journal
1040-4651
Volume
6
Issue
8
Year of publication
1994
Pages
1035 - 1047
Database
ISI
SICI code
1040-4651(1994)6:8<1035:ANEPFT>2.0.ZU;2-E
Abstract
A cDNA clone representing a novel cell wall protein was isolated from a tomato cDNA library. The deduced amino acid sequence shows that the encoded protein is very small (88 amino acids), contains an N-terminal hydrophobic signal peptide, and is enriched in lysine and tyrosine. W e have designated this protein TLRP for tyrosine- and lysine-rich prot ein. RNA gel blot hybridization identified TLRP transcripts constituti vely present in roots, stems, and leaves from tomato plants. The encod ed protein seems to be highly insolubitized in the cell wall, and we p resent evidence that this protein is specifically localized in the mod ified secondary cell walls of the xylem and in cells of the sclerenchy ma, In addition, the protein is localized in the protective periderm l ayer of the growing root. The highly localized deposition in cells des tined to give support and protection to the plant indicates that this cell wall protein alone and/or in collaboration with other cell wall s tructural proteins may have a specialized structural function by mecha nically strengthening the walls.