ALTERNATE POLYADENYLATION IN RODENT CELLS RESULTS IN 2 DIFFERENTIALLYEXPRESSED CYCLIN-B1 MESSENGER-RNAS

Citation
A. Maity et al., ALTERNATE POLYADENYLATION IN RODENT CELLS RESULTS IN 2 DIFFERENTIALLYEXPRESSED CYCLIN-B1 MESSENGER-RNAS, Biochemical and biophysical research communications, 202(2), 1994, pp. 908-914
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006-291X
Volume
202
Issue
2
Year of publication
1994
Pages
908 - 914
Database
ISI
SICI code
0006-291X(1994)202:2<908:APIRCR>2.0.ZU;2-X
Abstract
Cyclin B1 is required for the G2/M transition. In HeLa cells, the leve l of both its protein and mRNA varies dramatically through the cell cy cle. In this study we examine the expression of cyclin B1 mRNA in Chin ese hamster ovary cells and 3.7, a transformed rat embryo fibroblast l ine. In contrast to HeLa cells in which a single transcript is seen, b oth of these lines express two distinct transcripts of approximately 1 .4 and 2.7 kbp in length which appear to be made using different polya denylation sites. In CHO cells, the level of the upper band varies mod estly through the cell cycle while the lower band remains almost const ant. In contrast to HeLa cells, CHO cells in G1 express a significant amount of cyclin B1 mRNA. In synchronized 3.7 cells, the level of both transcripts is very low in G1 and rises in parallel as cells progress through S and G2, but the longer transcript disappears faster as cell s reenter G1. (C) 1994 Academic Press, Inc.