ELIMINATION OF TWINNING IN CRYSTALS OF SULFOLOBUS-SOLFATARICUS ALCOHOL-DEHYDROGENASE HOLO-ENZYME BY GROWTH IN AGAROSE GELS

Citation
F. Sica et al., ELIMINATION OF TWINNING IN CRYSTALS OF SULFOLOBUS-SOLFATARICUS ALCOHOL-DEHYDROGENASE HOLO-ENZYME BY GROWTH IN AGAROSE GELS, Acta crystallographica. Section D, Biological crystallography, 50, 1994, pp. 508-511
Citations number
11
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Crystallography,Biology,"Pharmacology & Pharmacy
ISSN journal
0907-4449
Volume
50
Year of publication
1994
Part
4
Pages
508 - 511
Database
ISI
SICI code
0907-4449(1994)50:<508:EOTICO>2.0.ZU;2-L
Abstract
Crystals of the binary complex of alcohol dehydrogenase from Sulfolobu s solfataricus with NADH were shown to be twinned and not suitable for automated data collection. Several crystallization trials, performed with the aim of eliminating twinning, are described. Interestingly, cr ystals grown from agarose gel have been demonstrated to have a unique reciprocal lattice. These crystals are monoclinic, space group C2, wit h cell dimensions a = 134.47 (9), b = 85.26 (5), c = 71.76 (8) angstro m, beta = 97.53 (4)-degrees, and showed significant diffraction beyond 3.0 angstrom resolution.