SPECIFIC EXPRESSION OF CPP32 IN SENSORY NEURONS OF MOUSE EMBRYOS AND ACTIVATION OF CPP32 IN THE APOPTOSIS INDUCED BY A WITHDRAWAL OF NGF

Citation
T. Mukasa et al., SPECIFIC EXPRESSION OF CPP32 IN SENSORY NEURONS OF MOUSE EMBRYOS AND ACTIVATION OF CPP32 IN THE APOPTOSIS INDUCED BY A WITHDRAWAL OF NGF, Biochemical and biophysical research communications, 231(3), 1997, pp. 770-774
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006-291X
Volume
231
Issue
3
Year of publication
1997
Pages
770 - 774
Database
ISI
SICI code
0006-291X(1997)231:3<770:SEOCIS>2.0.ZU;2-I
Abstract
We isolated mouse CPP32/apopain cDNA, a mammalian homologue most close ly related to Ced-3 in C. elegans, and examined the involvement of CPP 32 isl the apoptosis of nervous system during development. CPP32 is sp ecifically expressed in the trigeminal (V) ganglia, facio-acoustic (VI I-VIII) ganglion complex, and dorsal root ganglia (DRGs) of mouse 10.5 -day embryos, CPP32-like proteases are activated during apoptosis of D RG neurons induced by deprivation of NGF and serum. Ac-DEVD-CHO, an in hibitor for CPP32-like proteases, prevents apoptosis of DRG neurons, b ut Ac-YVAD-CHO, an inhibitor for ICE-like proteases, does not. These r esults suggest that CPP32 or CPP32-like proteases play a sole as centr al mediator in the apoptosis of DRG neurons induced by lack of neurotr ophin signals. (C) 1997 Academic Press.