RIBONUCLEASE-A CAN BE TRANSFORMED INTO A DIMERIC RIBONUCLEASE WITH ANTITUMOR-ACTIVITY

Citation
A. Didonato et al., RIBONUCLEASE-A CAN BE TRANSFORMED INTO A DIMERIC RIBONUCLEASE WITH ANTITUMOR-ACTIVITY, The Journal of biological chemistry, 269(26), 1994, pp. 17394-17396
Citations number
16
Language
INGLESE
art.tipo
Note
Categorie Soggetti
Biology
Journal title
ISSN journal
0021-9258
Volume
269
Issue
26
Year of publication
1994
Pages
17394 - 17396
Database
ISI
SICI code
0021-9258(1994)269:26<17394:RCBTIA>2.0.ZU;2-B
Abstract
A cDNA coding for bovine pancreatic RNase A was mutagenized to insert a proline, a leucine, and 2 cysteine residues, i.e. the residues prese nt at corresponding positions in the subunit of seminal RNase, the onl y dimeric RNase of the pancreatic-type superfamily, The mutant, expres sed in Escherichia coli, eventually aggregated into catalytically acti ve dimers. Like naturally dimeric seminal RNase, at equilibrium the mu tant dimeric RNase A adopted two quaternary structures (one with an ex change of the N-terminal segments between partner subunits, the other with no exchange) and displayed a selective toxicity for malignant cel ls, absent in the monomeric, parent protein.