MUTATIONS IN PTS CAUSE CATABOLITE-RESISTANT SPORULATION AND ALTERED REGULATION OF SPO0H IN BACILLUS-SUBTILIS

Authors
Citation
D. Frisby et P. Zuber, MUTATIONS IN PTS CAUSE CATABOLITE-RESISTANT SPORULATION AND ALTERED REGULATION OF SPO0H IN BACILLUS-SUBTILIS, Journal of bacteriology, 176(9), 1994, pp. 2587-2595
Citations number
64
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ISSN journal
0021-9193
Volume
176
Issue
9
Year of publication
1994
Pages
2587 - 2595
Database
ISI
SICI code
0021-9193(1994)176:9<2587:MIPCCS>2.0.ZU;2-Q
Abstract
A mutation in Bacillus subtilis, ggr-31, that relieves glucose-glutami ne-dependent control of a spoVG-lacZ translational fusion was isolated and was subsequently found to confer a pleiotropic phenotype. Mutants cultured in glucose- and glutamine-rich media exhibited a Crs(-) (cat abolite-resistant sporulation) phenotype; enhanced expression of the s poOH gene, encoding sigma(H), as evidenced by immunoblot analysis with anti-sigma(H) antiserum; and derepression of srfA, an operon involved in surfactin biosynthesis and competence development. In addition, gg r-31 mutants exhibited a significant increase in generation time when they were cultured in minimal glucose medium. The mutant phenotype was restored to the wild type by Campbell integration of a plasmid contai ning part of the ptsG (encoding the enzyme II/III glucose permease) ge ne, indicating that the mutation probably resides within ptsG and adve rsely affects glucose uptake. A deletion mutation within ptsI exhibite d a phenotype similar to that of ggr-31.