ASCORBIC-ACID, BUT NOT GLUTATHIONE, IS TAKEN UP BY BRAIN-SLICES AND PRESERVES CELL MORPHOLOGY

Citation
Me. Rice et al., ASCORBIC-ACID, BUT NOT GLUTATHIONE, IS TAKEN UP BY BRAIN-SLICES AND PRESERVES CELL MORPHOLOGY, Journal of neurophysiology, 71(4), 1994, pp. 1591-1596
Citations number
43
Language
INGLESE
art.tipo
Note
Categorie Soggetti
Neurosciences,Physiology
Journal title
ISSN journal
0022-3077
Volume
71
Issue
4
Year of publication
1994
Pages
1591 - 1596
Database
ISI
SICI code
0022-3077(1994)71:4<1591:ABNGIT>2.0.ZU;2-0
Abstract
We have determined the ascorbic acid(ascorbate) and glutathione (GSH) content of cortical and hippocampal slices from rat brain after prolon ged(6 h) incubation and have correlated these levels with the histolog ical quality of the slices. Ascorbate and GSH levels in control and sl iced tissue were determined by high performance liquid chromatography (HPLC) with electrochemical detection. Cell morphology of incubated sl ices was compared with that of intact tissue in cresyl violet stained tissue sections. Roughly 70% of tissue ascorbate and GSH was lost from slices during incubation in vitro. Normal in vivo levels of ascorbate (2-3 mu mol g(-1) tissue wet weight) could be maintained by including 200-400 mu M ascorbate (typical extracellular concentration) in the i ncubation media. By contrast, the loss of GSH could not be prevented b y incubation with GSH. The morphology of cells in hippocampal slices i ncubated under conditions that maintained ascorbate content and compar tmentalization were similar to those of intact tissue. Ascorbate prote cted pyramidal cells in CA1 and CA3 regions of the hippocampus from th e degeneration that was seen in slices incubated in ascorbate-free med ia. These data suggest that loss of endogenous antioxidants may be a m ajor factor in neuronal loss in vitro and support the notion that asco rbate is an endogenous neuroprotective agent.