DETECTION OF VIRULENT SHIGELLA AND ENTEROINVASIVE ESCHERICHIA-COLI BYINDUCTION OF THE 43 KDA INVASION PLASMID ANTIGEN, IPAC

Citation
R. Shamlal et al., DETECTION OF VIRULENT SHIGELLA AND ENTEROINVASIVE ESCHERICHIA-COLI BYINDUCTION OF THE 43 KDA INVASION PLASMID ANTIGEN, IPAC, FEMS immunology and medical microbiology, 17(2), 1997, pp. 73-78
Citations number
11
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology,Microbiology
ISSN journal
0928-8244
Volume
17
Issue
2
Year of publication
1997
Pages
73 - 78
Database
ISI
SICI code
0928-8244(1997)17:2<73:DOVSAE>2.0.ZU;2-U
Abstract
The invasion plasmid antigen, ipaC (43 kDa) of Shigella spp, and enter oinvasive Escherichia coli (EIEC) could be induced in vitro by growing them in the presence of Congo red. An enzyme-linked immunosorbent ass ay (ELISA) using antibodies to the 43 kDa protein of Shigella has been developed for specific detection of virulent Shigella spp and EIEC. T he test is independent of initial isolation of individual colonies. As few as 10(2) CFU/ml of virulent Shigella present in mixed cultures co uld be detected and concurrently their susceptibility to antibiotics c ould be analysed after an initial growth of 8-16 h in Congo red-contai ning medium, The test may prove useful in the diagnosis and treatment of bacillary dysentery caused either by Shigella or EIEC through their rapid identification and proper antimicrobial therapy.