AN ENZYMATIC ASSAY OF INORGANIC-PHOSPHATE IN SERUM USING NUCLEOSIDE PHOSPHORYLASE AND XANTHINE-OXIDASE

Citation
Jpj. Ungerer et al., AN ENZYMATIC ASSAY OF INORGANIC-PHOSPHATE IN SERUM USING NUCLEOSIDE PHOSPHORYLASE AND XANTHINE-OXIDASE, Clinica chimica acta, 223(1-2), 1993, pp. 149-157
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry Medicinal
Journal title
ISSN journal
0009-8981
Volume
223
Issue
1-2
Year of publication
1993
Pages
149 - 157
Database
ISI
SICI code
0009-8981(1993)223:1-2<149:AEAOII>2.0.ZU;2-R
Abstract
We have developed a new enzymatic assay for the determination of inorg anic phosphate (Pi) in serum, using nucleoside phosphorylase (NP) and xanthine oxidase (XOD). Pi and inosine react to form hypoxanthine and ribose-l-phosphate. The hypoxanthine is oxidized to xanthine, which is further oxidized to uric acid. In these two reactions 2,6-dichlorophe nolindophenol (DCIP) is reduced to a colourless compound and the decre ase in colour is measured spectrophotometrically at 600 nm. The assay is automated with an RA-XT analyser. The precision of the automated as say is acceptable (C.V. < 3.5%) and results are accurate and linear ac ross a range of values from 0.2-2.5 mmol/l. The assay correlates well with molybdate methods carried out on SMAC III and RA-XT analysers (r values 0.99 and 0.98, respectively), and seems to be less prone to non -specific sample interference than the usual RA-XT method. The enzymat ic assay described seems to be suitable for the routine determination of serum Pi.