CHOLESTEROL IN REMNANT-LIKE LIPOPROTEINS IN HUMAN SERUM USING MONOCLONAL ANTI APO B-100 AND ANTI APO A-I IMMUNOAFFINITY MIXED GELS

Citation
K. Nakajima et al., CHOLESTEROL IN REMNANT-LIKE LIPOPROTEINS IN HUMAN SERUM USING MONOCLONAL ANTI APO B-100 AND ANTI APO A-I IMMUNOAFFINITY MIXED GELS, Clinica chimica acta, 223(1-2), 1993, pp. 53-71
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry Medicinal
Journal title
ISSN journal
0009-8981
Volume
223
Issue
1-2
Year of publication
1993
Pages
53 - 71
Database
ISI
SICI code
0009-8981(1993)223:1-2<53:CIRLIH>2.0.ZU;2-W
Abstract
We have developed a simple, rapid assay method for apo E-rich lipoprot eins (d < 1.006 g/ml), using an immunoaffinity gel mixture of anti apo B-100 and apo A-I antibodies coupled to Sepharose 4B. The immunoaffin ity mixed gels adsorb normal lipoproteins containing apo A-I quantitat ively as well as most lipoproteins containing apo B-100. Unbound lipop roteins are quantified by assay of cholesterol. Characterization of th e unbound lipoproteins of d < 1.006 g/ml (J Lipid Res 1992;33:369-380) has shown that they represent chylomicron and VLDL remnant-like parti cles (RLP). RLP-Cholesterol(C) levels in plasma have been determined i n 363 male and female normolipidemic subjects (mean +/- S.D.: 72 +/- 1 6 mg/l) and have been found to be higher in patients with coronary hea rt disease and familial dysbetalipoproteinemia. Triglyceride-rich lipo proteins may well contain both atherogenic and nonatherogenic particle s that can be separated by this simple immunoadsorption assay.