IMMUNONEPHELOMETRIC TURBIDIMETRIC APOLIPOPROTEIN-B ASSAYS FOR THE CLINICAL LABORATORY/

Citation
Ss. Levinson et Sg. Wagner, IMMUNONEPHELOMETRIC TURBIDIMETRIC APOLIPOPROTEIN-B ASSAYS FOR THE CLINICAL LABORATORY/, Clinica chimica acta, 223(1-2), 1993, pp. 31-42
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry Medicinal
Journal title
ISSN journal
0009-8981
Volume
223
Issue
1-2
Year of publication
1993
Pages
31 - 42
Database
ISI
SICI code
0009-8981(1993)223:1-2<31:ITAAFT>2.0.ZU;2-I
Abstract
Because apolipoproteins are a part of complex macromolecular particles , modifications to the assay system may substantially after results of immunological measurement. Accuracy as analytical recovery cannot be effectively determined by adding exogenous apolipoproteins because ant ibody access differs from access to endogenous apolipoproteins. Clinic al studies are essential for determining accuracy in terms of clinical effectiveness. Since different kit methods use different reagent syst ems, the purpose of the present study was to compare total cholesterol and LDL cholesterol as markers for coronary artery disease with apo B by automated rate nephelometric, end-point nephelometric and turbidim etric kit methods. The subjects were age matched, male patients with a nd without angiographically documented coronary artery disease. High c orrelation coefficients (0.95-0.96) between the assays for both the no rmal and disease groups indicate that the methods are providing simila r information: apo B was a better marker for coronary artery disease ( CAD) than total or LDL cholesterol on the basis of univariate, multiva riate and Bayesian statistics and correlated best with non-HDL cholest erol. Apo B along with HDLC could explain the variability between the CAD and normal groups without LDLC, total C, or triglycerides.