COMPARATIVE DRUG INFLUENCE ON PEROXIDE MEDIATED INCREASE OF CYTOSOLICCALCIUM IN HUMAN ENDOTHELIAL-CELLS

Citation
M. David et al., COMPARATIVE DRUG INFLUENCE ON PEROXIDE MEDIATED INCREASE OF CYTOSOLICCALCIUM IN HUMAN ENDOTHELIAL-CELLS, Clinica chimica acta, 223(1-2), 1993, pp. 1-7
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry Medicinal
Journal title
ISSN journal
0009-8981
Volume
223
Issue
1-2
Year of publication
1993
Pages
1 - 7
Database
ISI
SICI code
0009-8981(1993)223:1-2<1:CDIOPM>2.0.ZU;2-M
Abstract
The increase of cytosolic free calcium in human umbilical vein endothe lial cells caused by peroxides was used as a model to determine and co mpare the putative cytoprotective properties of substances known to in terfere with the generation or metabolism of reactive oxygen species. Hydrophilic hydrogen peroxide and lipophilic cumene hydroperoxide were used as sources of reactive oxygen. [Ca2+](i) in endothelial cells wa s measured by the FURA method and the resting level was found to be 12 9.3 +/- 14.1 nM. Both peroxides were found to increase cytosolic calci um with dependence on the concentration and on the presence of extrace llular calcium. Among the substances tested, only catalase and N-acety l-cysteine were able to exhibit a significant cytoprotective effect.