Xz. Zhang et T. Komatsuada, PLANT-REGENERATION FROM SOYBEAN (GLYCINE-MAX L) PROTOPLASTS VIA SOMATIC EMBRYOGENESIS, Science in China. Series B, Chemistry, life sciences & earth sciences, 36(12), 1993, pp. 1476-1482
Protoplasts isolated f rom immature cotyledons of cultivated soybean (
Glycine max L.) were cultured by Gelrite bead method in ZSP medium wit
h soybean root nodule products (asparagine, glutamine, allantoin, alla
ntoic acid, etc.) as the main nitrogen resources. Embryogenic calli we
re initiated and transferred to somatic embryo differentiation medium.
Somatic embryos were formed from embryogenic calli and converted to p
lants. The regenerated plants normally grew and flowered.