MONITORING THE NATURAL COURSE AND RESPONSE TO THERAPY OF CHRONIC HEPATITIS-B WITH AN AUTOMATED SEMIQUANTITATIVE ASSAY FOR IGM ANTI-HBC

Citation
Mr. Brunetto et al., MONITORING THE NATURAL COURSE AND RESPONSE TO THERAPY OF CHRONIC HEPATITIS-B WITH AN AUTOMATED SEMIQUANTITATIVE ASSAY FOR IGM ANTI-HBC, Journal of hepatology, 19(3), 1993, pp. 431-436
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Gastroenterology & Hepatology
Journal title
ISSN journal
0168-8278
Volume
19
Issue
3
Year of publication
1993
Pages
431 - 436
Database
ISI
SICI code
0168-8278(1993)19:3<431:MTNCAR>2.0.ZU;2-D
Abstract
The clinical significance of a semi-quantitiative microparticle enzyme immunoassay (IMx Core-M, Abbott) was evaluated for detection of IgM-c lass antibodies against the hepatitis B core antigen (IgM anti-HBc) in 136 hepatitis B surface antigen (HBsAg) positive individuals (96 chro nic HBV carriers, 20 patients with chronic HBV-HDV infections and 20 p atients with acute hepatitis B) and 50 HBV-negative controls. Baseline and follow-up sera (4-11 samples) were analysed from 79 carriers with chronic hepatitis B, 44 of whom were treated with interferon. IMx ind exes above 3.000 were found in 95% of the acute hepatitis B patients a nd above 0.300 in 91.5% of patients with ongoing chronic hepatitis B. IMx indexes between 0.200 and 0.300 were observed in (a) patients with recent HBeAg to anti-HBe seronconversion (6-12 months) and normal ser um ALT levels, (b) patients immune-tolerant to HBV infection and witho ut liver disease despite high levels of viremia, and (c) patients with anti-HBe-positive chronic hepatitis B during 7-13-month intervals of asymptomatic carriage between episodes of disease reactivation. IMx in dexes below 0.200 were detected in all HBV-negative individuals and he althy HBV carriers, in 14 (70%) of 20 chronic hepatitis D patients and in all but 1 of 22 interferon-treated patients with histological remi ssion of liver disease, 5-12 months after clearance of viremia and nor malization of serum ALT levels. In contrast, IMx indexes remained abov e 0.200 in all patients with hepatitis B reactivation. These results s uggest that the semi-quantitative analysis of serum IgM anti-HBc is a useful non-invasive tool for the diagnosis and monitoring of chronic h epatitis B. In patients with undetectable viremia and normal serum ami notransferases levels, an IgM anti-HBc index below 0.200(<10 PEI units ) is associated with persistent remission of liver disease while value s above 0.200 represent the hallmark of hepatitis B reactivation.