NERVE GROWTH-FACTOR MESSENGER-RNA IS EXPRESSED BY GABAERGIC NEURONS IN RAT HIPPOCAMPUS

Citation
Jc. Lauterborn et al., NERVE GROWTH-FACTOR MESSENGER-RNA IS EXPRESSED BY GABAERGIC NEURONS IN RAT HIPPOCAMPUS, NeuroReport, 5(3), 1993, pp. 273-276
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0959-4965
Volume
5
Issue
3
Year of publication
1993
Pages
273 - 276
Database
ISI
SICI code
0959-4965(1993)5:3<273:NGMIEB>2.0.ZU;2-8
Abstract
ISOTOPIC and colormetric in situ hybridization techniques were combine d to determine if nerve growth factor (NGF) mRNA is colocalized with m RNA for the GABA biosynthetic enzyme glutamic acid decarboxylase (GAD) in adult rat hippocampus. Quantification of neurons labeled with both S-35-labeled GAD(67) mRNA and digoxigenin-labeled NGF cRNA determined that of the NGF cRNA-labeled neurons, 97% within regions CA3-CA1, and 88% within the hilus, were also labeled with GAD(67) cRNA. Overall, 4 7% of the total population of GAD(67) cRNA labeled cells were NGF cRNA positive. The greater portion of stratum granulosum was lightly label ed by the NGF cRNA alone. The results indicate that, excepting stratum granulosum, NGF is predominantly synthesized by GABAergic neurons in rat hippocampus.