CAMP-INDUCED CHANGES OF INTRACELLULAR FREE MG2+ LEVELS IN HUMAN ERYTHROCYTES

Citation
T. Matsuura et al., CAMP-INDUCED CHANGES OF INTRACELLULAR FREE MG2+ LEVELS IN HUMAN ERYTHROCYTES, Biochimica et biophysica acta, 1220(1), 1993, pp. 31-36
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biophysics,Biology
ISSN journal
0006-3002
Volume
1220
Issue
1
Year of publication
1993
Pages
31 - 36
Database
ISI
SICI code
0006-3002(1993)1220:1<31:CCOIFM>2.0.ZU;2-#
Abstract
To examine the role of cAMP in the regulation of intracellular free ma gnesium concentration ([Mg2+]i), we measured [Mg2+]i in human erythroc ytes by P-31-NMR spectroscopy. (-)-Isoproterenol, forskolin, Bt2cAMP a nd 8-bromo-cAMP decreased [Mg2+]i in human erythrocytes. Bt2cAMP did n ot increase the effux rate of Mg2+ from erythrocytes. HA1004, a potent inhibitor of cAMP-dependent kinases, markedly increased the [Mg2+]i i n a Mg2+-free buffer solution. Addition of 8-bromo-cGMP or 12-O-tetrad ecanoylphorbol 13-acetate (TPA) did not affect the [Mg2+]i. These resu lts suggest that beta-adrenergic stimulation and cAMP play an importan t role in the regulation of [Mg2+]i in human erythrocytes.