The complete nucleotide sequences of 23S rRNA genes from Bradyrhizobiu
m, japonicum DSM 30131 and from closely related Rhodopseudomonas palus
tris DSM 126 were determined. A specific hybridization probe for Brady
rhizobium japonicum was designed and tested. The probe hybridized to n
ucleic acids from all Bradyrhizobium japonicum strains tested and did
not react with nucleic acids from other reference bacteria.