IN-SITU IDENTIFICATION OF LACTOCOCCI, ENTEROCOCCI AND STREPTOCOCCI

Citation
C. Beimfohr et al., IN-SITU IDENTIFICATION OF LACTOCOCCI, ENTEROCOCCI AND STREPTOCOCCI, Systematic and applied microbiology, 16(3), 1993, pp. 450-456
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0723-2020
Volume
16
Issue
3
Year of publication
1993
Pages
450 - 456
Database
ISI
SICI code
0723-2020(1993)16:3<450:IIOLEA>2.0.ZU;2-0
Abstract
The genera Lactococcus, Enterococcus, and Streptococcus contain specie s of biotechnological and medical importance. The need for rapid and r eliable identification of isolates has prompted the development of sev eral 16S and 23S rRNA based oligonucleotide probes. Fluorescent deriva tives of probes specific for Lactococcus lactis, L. lactis subsp. crem oris, Enterococcus faecalis, E. faecium, Streptococcus salivarius and S. thermophilus were used for whole cell hybridization. Optimal probe permeability of fixed cells required special pretreatment of these Gra m-positive organisms. Fluorescent probing in combination with epifluor escence microscopy resulted in single cell identification. With this a ssay lactococci, enterococci, and streptococci can be identified in mi lk samples within one day.