METHANOBACTERIUM THERMOFLEXUM SP-NOV AND METHANOBACTERIUM DEFLUVII SP-NOV, THERMOPHILIC ROD-SHAPED METHANOGENS ISOLATED FROM ANAEROBIC DIGESTER SLUDGE

KOTELNIKOVA SV OBRAZTSOVA AY GONGADZE GM LAURINAVICHIUS KS

Sv. Kotelnikova et al., METHANOBACTERIUM THERMOFLEXUM SP-NOV AND METHANOBACTERIUM DEFLUVII SP-NOV, THERMOPHILIC ROD-SHAPED METHANOGENS ISOLATED FROM ANAEROBIC DIGESTER SLUDGE, Systematic and applied microbiology, 16(3), 1993, pp. 427-435

29

INGLESE

Article

Microbiology,"Biothechnology & Applied Migrobiology

Systematic and applied microbiology → ACNP

0723-2020

16

3

1993

427 - 435

ISI

0723-2020(1993)16:3<427:MTSAMD>2.0.ZU;2-E

Two new rod-shaped Gram-positive, autotrophic, thermophilic methanogen s, strains ADZ and IDZ, were isolated from the anaerobic digestor slud ge operating on waste waters containing methacrylates. The isolates us e H-2 + CO2 and formate as substrates for growth and methane productio n. Strain ADZ grows with a doubling time of 1.5 h under optimal condit ions (60 degrees C, pH 7.0, 25 mg/l Na2S, 0.8-20 g/l NaCl). The GC con tent of the DNA is 62.2 mol%. Strain IDZ grows with a doubling time of 3.5 h under optimal conditions (55 degrees C, pH 7.9-8.2, 150 mg/l Na 2S, 1-30 g/l NaCl). The GC content of the DNA for the strain IDZ is 55 mol%. The new isolates are closely related to the genus Methanobacter ium, but differ from each other and from the members of the genus ment ioned antigenically, in amino acid composition of cell walls, low DNA- DNA homology and the GC content of the DNA. Therefore, they are consid ered as a new species of the genus Methanobacterium, which we name Met hanobacterium thermoflexum with type strain IDZ (VKM B-1963), and Meth anobacterium, defluvii with type strain ADZ (VKM B-1962).