INTRASPECIFIC VARIABILITY OF XANTHOMONAS ALBILINEANS

Citation
P. Yang et al., INTRASPECIFIC VARIABILITY OF XANTHOMONAS ALBILINEANS, Systematic and applied microbiology, 16(3), 1993, pp. 420-426
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0723-2020
Volume
16
Issue
3
Year of publication
1993
Pages
420 - 426
Database
ISI
SICI code
0723-2020(1993)16:3<420:IVOXA>2.0.ZU;2-X
Abstract
Sixty five Xanthomonas albilineans strains originating from 22 countri es were analysed by sodium dodecyl sulfate-polyacrylamide gel electrop horesis (SDS-PAGE) of whole-cell proteins, indirect-immunofluorescent antibody typing and gas-liquid chromatographic analysis of fatty acid methyl esters (FAMEs). The strains were separated into two major SDS-P AGE protein clusters, three serovars and three FAME groups. SDS-PAGE p rotein cluster 1 contained 48 strains which represented a world-wide g eographic distribution (with the exception of Fiji and tropical Africa ), the majority of which (47) belonged to serovar 1. SDS-PAGE protein cluster 2 comprised 17 strains mainly from tropical Africa and Fiji, w hich belonged either to serovars 2 or 3 (except strain LMG 478). There was less correlation between the grouping based on FAME composition a nd those based on SDS-PAGE protein profiles or serological properties. The genomic relationship between X. albilineans and other Xanthomonas species was investigated by DNA:DNA hybridization. A DNA relatedness of 97% was found within X. albilineans, whereas low DNA hybridization values (6-37%) were observed between X. albilineans type strain LMG 49 4 and representative strains from the other 20 Xanthomonas DNA hybridi zation groups so far determined. Within the genus Xanthomonas, X. albi lineans clearly constitutes a well-delineated species.